Optionally, repeat the elution step, and incubate the spin column on the bench for 10 minutes with RNase-free water before centrifuging.

The PureLink™ HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients.

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The customer is required to ensure that the use of any products supplied by VWR does not infringe the intellectual property rights of any third party and the customer shall indemnify VWR against any claims made against VWR by any third party in relation to any such infringement or alleged infringement. Total RNA purified with the RNeasy Maxi Kit is of high quality and is suitable for many downstream applications (see figure "High-quality RNA from a variety of samples"). Total RNA is easily purified with the RNeasy Maxi Kit from large amounts of starting material including animal or human cells, animal or human tissues, and yeast cells (see table “Total RNA yields obtained with RNeasy Kits”). The dedicated RNeasy Mini QIAcube Kit, including rotor adapters preloaded with RNeasy spin columns and elution tubes, delivers greater convenience and time savings (see figure "Significant time savings with dedicated QIAcube Kits").

Customers who exceed their credit limits will be asked to pay in advance for additional products and/or services until the account is settled.Where delivery or performance dates are stated by VWR these are estimates only and time is not of the essence; however, if VWR needs to change such dates it will do so only after providing information to the customer and having regards to the customer’s stated objectives. Authorisation will be subject to the condition that the products are returned to VWR Customer Service Centre or to the manufacturer or other source and by the method advised by VWR.