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Shocktato Party Game - The Hilariously Funny Game of Shocking Potato

£9.9£99Clearance
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N1 - This work was funded by the BBSRC grant (BB/M004899/1) as part of the ERA-CAPS project HotSol and the Scottish Government Rural and Environment Science and Analytical Services Division as part of the Strategic Research Programme 2016-2021. Mardis ER (2008) Next-generation DNA sequencing methods. Annu Rev Genomics Hum Genet 9:387–402. https://doi.org/10.1146/annurev.genom.9.081307.164359

Alexa A, Rahnenfuhrer J, Lengauer T (2006) Improved scoring of functional groups from gene expression data by decorrelating GO graph structure. Bioinformatics 22:1600–1607. https://doi.org/10.1093/bioinformatics/btl140

Lichtenthaler HK, Wellburn AR. Determination of total caroteonids and chlorophyll a and b of leaves and different solvents. Biochem Soc Trans. 1983; 11(5):591–592. doi: 10.1042/bst0110591. [ CrossRef] [ Google Scholar] Schramm F, Ganguli A, Kiehlmann E, Englich G, Walch D, Koskull-Döring PV. The heat stress transcription factor Hsfa2 serves as a regulatory amplifier of a subset of genes in the heat stress response in Arabidopsis. Plant Mol Biol. 2006;53(5):264.

Rykaczewska K (2013) The impact of high temperature during growing season on potato cultivars with different response to environmental stresses. Am J Plant Sci 4:2386–2393. https://doi.org/10.4236/ajps.2013.412295DIRECTIONS - Turn it on with the on button. Then choose the game mode and hit start. Toss it around to see who has the best reaction to the jolt and get ready to laugh. A major difficulty in screening for complex abiotic stress tolerance traits is control of environmental parameters whilst growing sufficient numbers of genotypes under replication for genetic analysis. In potato, segregation for earliness of tuberization is a confounding factor in heat stress screening and has led to the development of screens based on nodal cuttings (Ewing and Wareing, 1978; Van den Berg etal., 1990). This technique uses an excised potato leaf and its subtended axillary bud, that when maintained in moist compost, tuberizes rapidly. Leaf cuttings from induced plants produce tubers (in ~14days) at the axillary bud, whereas non‐induced plants fail to tuberize. The nodal cutting assay therefore provides a convenient, space and time‐saving high‐throughput method to study tuberization and dry matter partitioning. The use of a model system for analysis of traits that are impacted on by multiple factors has the potential to simplify the trait and allows the investigator to focus on specific components. Hsp70 chaperone dynamics and molecular mechanism. Trends Biochem. Sci. 38, 507–514. [ PubMed] [ Google Scholar]

Gu Z, Cavalcanti A, Chen FC, Bouman P, Li WH. Extent of gene duplication in the genomes of drosophila, nematode, and yeast. Mol Biol Evol. 2002;19(3):256–62. Wang W, Vinocur B, Altman A. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta. 2003;218(1):1–14. Primer Premier 5 was used to design primers specific to the StHsp20 genes (Additional file 3: Table S3). Total RNA was extracted using an RNAsimple Total RNA Kit (BioTeke, Beijing, China). The cDNA was reverse-transcribed by First Strand cDNA Synthesis Kit, ReverTra Ace-α (TOYOBO, Shanghai, China). All of the operational procedures followed the manufacturer’s protocols. Before the qRT-PCR analysis, 1 μl cDNA was diluted with 4 μl nuclease-free water.

etal (2008) Phylogeny. fr: robust phylogenetic analysis for the non‐specialist. Nucleic Acids Res. 36, W465–W469. [ PMC free article] [ PubMed] [ Google Scholar] Deng W, Wang Y, Liu Z, Cheng H, Xue Y. Hemi: a toolkit for illustrating heatmaps. PLoS One. 2013;9(11):e111988. All of the high-confidence Hsp20 sequences were submitted to ExPASy ( http://web.expasy.org/protparam/) to calculate the number of amino acids, molecular weights and theoretical isoelectric points (pI). The chromosomal locations and intron numbers of StHsp20s were acquired through the PGSC. The MEME program (version 4.11.2, http://alternate.meme-suite.org/tools/meme) was used to identify the conserved motifs in the StHsp20s sequences, with the following parameters: any number of repetitions, maximum of 10 misfits and an optimum motif width of 6 - 200 amino acid residues. The exon–intron structures of the StHsp20 genes were identified on the Gene Structure Display Server (GSDS, http://gsds.cbi.pku.edu.cn/) [ 30]. Chromosomal localization and gene duplication Hijman JB. The effect of climate change on global potato production. Amer J Pot Res. 2003; 80:271–280. doi: 10.1007/BF02855363. [ CrossRef] [ Google Scholar]

Sources of heat tolerance amongst potato cultivars, breeding lines, and Solanum species. Euphytica 55, 235–245. [ Google Scholar] In earlier studies, Arabidopsis Hsp20 genes were classified into seven subfamilies (CI, CII, CIII, M, P, ER and Po), and five genes could not be clustered into any subfamily [ 23]. Subsequently, four new nucleocytoplasmic subfamilies (CIV, CV, CVI and CVII) and a mitochondrial subfamily (MII) were identified [ 35]. In our study, the phylogenetic tree showed that Hsp20 genes were classified into 12 distinct subfamilies. The StHsp20 genes existed in 11 of the 12 subfamilies. There was no Hsp20 gene of potato in the CIV subfamily, which may be the result of gene loss during evolution. Yu J, Cheng Y, Feng K, Ruan M, Ye Q, Wang R, Li Z, Zhou G, Yao Z, Yang Y, Wan H. Genome-wide identification and expression profiling of tomato Hsp20 gene family in response to biotic and abiotic stresses. Front Plant Sci. 2016;7:1215. Cofactor Tpr2 combines two TPR domains and a J domain to regulate the Hsp70/Hsp90 chaperone system. EMBO J. 22, 3613–3623. [ PMC free article] [ PubMed] [ Google Scholar]Wang L, Guo K, Li Y, Tu Y, Hu H, Wang B, Cui X, Peng L. Expression profiling and integrative analysis of the cesa/csl superfamily in rice. BMC Plant Biol. 2010;10(1):282. Genetic variation for lettuce seed thermoinhibition is associated with temperature‐sensitive expression of abscisic acid, gibberellin, and ethylene biosynthesis, metabolism, and response genes. Plant Physiol. 148, 926–947. [ PMC free article] [ PubMed] [ Google Scholar]

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