Both Misty and Jessie were voiced by Rachael Lillis in the 4Kids dub, then they switched to Michele Knotz when TPCi took over. As Here's Lookin at You, Elekid was the first episode in the anime to feature digital colored animation, Jessie's hair color was changed to magenta in all subsequent episodes. In all of the hand-colored cel animated episodes prior to this point, including movies like Destiny Deoxys and Lucario and the Mystery of Mew, Jessie's hair was colored red.

During the Lumiose Conference, he along with Jessie, Meowth, and Wobbuffet serve as cameramen for one of the members of Elite Four and news reporter, Malva, and are unaware of Malva's alignment with Team Flare. But as Lysandre causes chaos through Lumiose City with Z-2 as Zygarde in its 50% form, James, Jessie, and Meowth had no choice but to help Professor Sycamore, Mairin, and Serena. With the assistance of the Hoenn Champion, Steven Stone, the trio finally remove their disguises and tells them to move on to get Chespie while facing off against Team Flare grunts including Celosia and Mable. Throughout these episodes, they are seen trying to steal others' Pokémon, including Pikachu and Pokémon that are able to Mega evolve. However, they are blasted off by Ash and his group or other Trainer's Pokémon . They also fight against the evil Malamar group on several occasions. Along with James and Meowth, Jessie reappeared in New Places... Familiar Faces!, returning to their original plan of catching Ash's Pikachu. They've also added new Pokémon to their team, with Jessie catching a Frillish sometime before meeting up with Ash and company. However, she is forced to give it and her Woobat away to Giovanni in The Dream Continues! under the pretense that she captured them for him. She also regained Wobbuffet as part of her team in the episode.It was revealed in The Sun, the Scare, the Secret Lair! that Jessie has a dislike for Giovanni's secretary Matori, who regularly displays a contemptuous and condescending attitude towards the trio and acts as if she is their boss. Jessie in turn sees Matori as a rival figure and even calls her "four eyes". In The Battle Of The Badge, Jessie, James, and Meowth were made the temporary Gym Leaders of the Viridian Gym in Giovanni's place. Having rigged the platforms so that the Trainer feels the pain their Pokémon feels, they battled against Ash. Despite using Giovanni's Pokémon and cheating, they ultimately lost and were sent blasting off. The trio later participates in their boss Giovanni's Operation Tempest in both Meloetta and the Undersea Temple! and Unova's Survival Crisis!. Jessie and James fought both Cilan and Iris using his Yamask and her Woobat against Pansage and Axew. They are amused to see their boss, Giovanni, defeat Ash and his Pikachu using Persian as his battling Pokémon as well as to capture Meloetta in their aircraft. Jessie and Meowth are also disappointed when James successfully catches Inkay by luring it in with food. Therefore, when she tripped into a wild Pumpkaboo, she decided to capture it.

Reason: Missing overworld and in-battle models and story info from LGPE; need higher-quality VS model from LGPEIn A Battle of Aerial Mobility!, Giovanni approved the trio's plan to steal as many rare and powerful Pokémon as they could in Kalos. Finally, at Silph Co. in Saffron City, Jessie and James make their final appearance, once again trying to stall the main character from reaching their Boss.

In Master Class Is in Session!, having gained the two Princess Keys needed to enter the Master Class, she entered the Gloire City Pokémon Showcase Master Class. In the first round, she performed against Nini and Concetta, where she dazzled the audience with Shadow Ball from her Gourgeist. When the second part of the first round began, she chose to strike a series of poses with her Gourgeist while Nini and Concetta appealed to the audience, but later joined the appeals, commanding Shadow Ball from her Gourgeist which enveloped the stage in a lilac smoke. Due to her appeals, she managed to advance to the next round in which she participated with Meowth, and made it through to the top three semi-finalists. Jessilee seemed pleased when Monsieur Pierre announced the rules for the semi-final round in the next episode, claiming she just has to do what she has been since doing. Despite putting up a good performance with Meowth and her Gourgeist, she was defeated, and despite her partners' fear, did not seem sad about it, instead being happy that Kalos could see her beauty. When the Master Class was over, she was seen walking through Gloire City rather silently, causing James, Meowth, Inkay and her Pokémon to worry and fear her wrath. However, she shrugged their fears and decided that she just wanted to give a feast to them, which shall be financed by James, for supporting her.Jessie returned once again in Pokémon the Series: XY, following Ash to the Kalos region. In Kalos, Jessie became a Pokémon Performer, competing under the aliases Jessilee and Jessilia (Japanese: ムサヴィ Musavie). Elena Ramírez ( EP159- EP167, EP169, EP192- EP205, EP230- EP233, EP274- AG017, AG033- AG034, AG093- AG100, one scene of AG133)